Lanes 1 3 and 5.
Cdk2 antibody cell signaling.
Mix well then add 0 1 g bsa mix.
Cyclin dependent kinase 2 p33cdk2 is an important component of the cell cycle machinery.
Red signal from loading control ab8226 observed at 42 kda lanes 5 and 6.
The antibody weakly cross reacts with cdc2 phosphorylated at thr161.
An antibody shouldn t be one of the variables in your experiment.
Anti rabbit igg h l f ab 2 fragment alexa fluor 488 conjugate 4412.
Immunoprecipitation of cdk12 from pc 3 cell extracts using normal rabbit igg 2729 lane 2 or cdk12 antibody lane 3.
Cdk2 knockout hap1 cell lysate 20 µg lanes 1 and 2.
Like p34cdc2 kinase activity is regulated by phosphorylation state as well as association with a cyclin subunit and a cdk inhibitor.
Inhibitory phosphorylation occurs on thr14 and tyr15 1.
Merged red and green signal.
Antibody dilution buffer 1x pbs 1 bsa 0 3 triton x 100.
Wild type hap1 cell lysate 20 µg lanes 2 4 and 6.
Ab32147 was shown to specifically react with cdk2 when cdk2 knockout samples were used.
Inhibitory phosphorylation occurs on thr14 and tyr15 1.
Green signal from target ab32147 observed at 34 kda lanes 3 and 4.
Kinase activity associated with cyclin a cdc2 is found only in g2 phase.
Cdk2 antibodies cdk2 antibodies p33cdk2 associates with cyclin a in human cells.
Cyclin dependent kinase 2 p33cdk2 is an important component of the cell cycle machinery.
Antibodies are purified by protein a and peptide affinity chromatography.
Western blot analysis was performed using cdk12 antibody.
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Inhibitory phosphorylation occurs on thr14 and tyr15 1.
Like p34cdc2 kinase activity is regulated by phosphorylation state as well as association with a cyclin subunit and a cdk inhibitor.
Phospho cdk2 thr160 antibody detects endogenous levels of cdk2 only when phosphorylated at threonine 160.
Like p34cdc2 kinase activity is regulated by phosphorylation state as well as association with a cyclin subunit and a cdk inhibitor.
It does not cross react with other phosphorylated cyclin dependent kinases.
Lane 1 is 10 input.
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding thr160 of human cdk2.
Cdk2 cell division protein kinase 2 cyclin dependent kinase 2 cdc2 related protein kinase cell devision kinase 2 p33 protein kinase p33 cdk2 immunogen.
