Cell Sorting Flow Cytometry

Michael timm s pearl on cell sorting using flow cytometry explains the origins of cell sorting technology the two main types and their differences as well as best practices and troubleshooting sorting assays.

Cell sorting flow cytometry.

12 14 31 in flow cytometers with sorting capabilities the instrument detects cells using parameters including cell size morphology and protein expression and then droplet technology. Download slides pdf transcript. Hello my name is michael timm. Cell sorting by flow cytometry cell sorting is a method to purify cell populations based on the presence or absence of specific physical characteristics.

Download transcript pdf slide 1. For example if lymphocytes were concentrated to 20 million per ml the flow rate at the instrument could be run as high as 20 000 cells per second. Researchers partnering with nyu langone s cytometry and cell sorting laboratory have access to the most powerful and adaptable flow cytometry and cell sorting technologies and instruments available. If your research requires cytometric analysis our state of the art instruments acquire optical measurements using different lasers to detect fluorophores with a high level of precision.

Forward scatter fsc forward light scatter refers to the refracted light past the cell into a detector behind the laser. The amount of refraction refers to the size and volume of the cell. Cells larger than lymphocytes require a larger nozzle to be placed on the instrument and the flow rate is lower. Cell sorting is the separation and isolation of various cell populations.

With flow cytometry cell sorting multiple detectors are being utilized to analyze different cell characteristics.